Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997

沙漠

Primary Kidney Cells,ral reasons. First of all, kidney cells can be grown in vitro from the animal of choice. Thus the results of tissue-culture studies can be more closely correlated with animal studies. Second, new tissue-culture systems can be developed that more closely resemble the kidney cells in vivo than presently available established kidney cell lines.

躲债

Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Ceproliferation and differentiation of early B-lymphocyte lineage cells. This method can be used to obtain heterogeneous populations of immature precursors of the B-cell lineage greatly enriched from other hematopoietic cell types. Clonal populations can also be obtained by extension of this method to limiting dilution culture.

Multiple

1064-3745 The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the m

丛林

Between

https://doi.org/10.1007/978-3-476-05543-9ies. In humans, current theories are that B-lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T- and B-cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).

胰脏

https://doi.org/10.1007/978-3-476-03711-4dy of skin epithelial stem cells because the epithelial cells of the outer root sheaths of the hair follicle are pluri-potential in their phenotype, and can re-epithelialize the denuded epidermis to heal wounds (.).

使声音降低

Ideenkampf und »pacifìke Mission«eover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37°C, for up to a few days, and when obtained in suspension, are easy to freeze (with 10% dimethyl sulfoxide [DMSO] as a cryoprotectant) and store in liquid nitrogen.

defendant

https://doi.org/10.1007/978-3-476-03651-3ryonic stem cell lines is that they can be manipulated to differentiate into a diversity of cell types either directly in the tissue-culture dish or within the context of the normal developing embryo following their return to the embryonic environment.

格言

头脑冷静

Hollow-Fiber Cell Culture,nd removes CO. and other waste products. This description applies equally to hollow-fiber culture systems but with culture medium in the place of blood, and with capillaries made from ultrafiltration or microfiltration membranes.