Titlebook: Basic Cell Culture Protocols; Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture.

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Hematopoietic Development of Human Embryonic Stem Cells in Culture,s of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.

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Cheryl D. Helgason,Cindy L. MillerIncludes supplementary material:

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180966.jpg

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https://doi.org/10.1385/1592598382DNA; Macrophages; cell; cell culture; cell lines; development; enzyme; human cell lines; neural stem cells; s

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Maiken Umbach,Claus-Christian W. Szejnmannuding the reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, time-lapse visualization of thymic emigration, reaggregation culture, and retrovirus-mediated gene transfer to developing thymocytes in FTOC.

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Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line, nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described.

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