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Titlebook: Basic Cell Culture Protocols; Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture.

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期刊全称Basic Cell Culture Protocols
影响因子2023Cheryl D. Helgason,Cindy L. Miller
视频video
发行地址Includes supplementary material:
学科分类Methods in Molecular Biology
图书封面Titlebook: Basic Cell Culture Protocols;  Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture.
影响因子In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).
Pindex Book 20053rd edition
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Ergebnisse des quantitativen Studienteils,eadily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a
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https://doi.org/10.1007/978-3-476-05768-6es. In this regard, it is crucial that the cells faithfully correspond to the purported objects of study. A number of recent publications have shown an unacceptable level of cell lines to be false, in part as a result of the nonavailability of a simple and easy DNA profiling technique. We have valid
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Maiken Umbach,Claus-Christian W. Szejnmannprogenitors that can be detected in vitro using colony-forming cell (CFC) assays. Hematopoietic progenitor cells, when cultured in a semisolid methylcellulose-based medium that is supplemented with suitable growth factors, proliferate and differentiate to produce clonal clusters (colonies) of maturi
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https://doi.org/10.1007/978-0-230-39111-6ly be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an a
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https://doi.org/10.1007/978-0-230-39111-6rations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be puri
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