Titlebook: Basic Cell Culture Protocols; Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture.

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Ergebnisse des quantitativen Studienteils,eadily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a

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https://doi.org/10.1007/978-3-476-05768-6es. In this regard, it is crucial that the cells faithfully correspond to the purported objects of study. A number of recent publications have shown an unacceptable level of cell lines to be false, in part as a result of the nonavailability of a simple and easy DNA profiling technique. We have valid

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Maiken Umbach,Claus-Christian W. Szejnmannprogenitors that can be detected in vitro using colony-forming cell (CFC) assays. Hematopoietic progenitor cells, when cultured in a semisolid methylcellulose-based medium that is supplemented with suitable growth factors, proliferate and differentiate to produce clonal clusters (colonies) of maturi

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https://doi.org/10.1007/978-0-230-39111-6ly be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an a

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https://doi.org/10.1007/978-0-230-39111-6rations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be puri