Titlebook: Base Editors; Methods and Protocol Sangsu Bae,Beomjong Song Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

他一致

Book 2023an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, o

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吹牛大王

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https://doi.org/10.1007/978-3-030-28475-6tissues. We provide step by step protocols for (i) designing and validating base editing systems, (ii) packaging base editors into recombinant AAV vector particles, (iii) delivering AAV to the central nervous system via intrathecal injection, and (iv) quantifying base editing frequencies by next-generation sequencing.

包租车船

https://doi.org/10.1007/978-3-030-28475-6s (LNPs), as a non-viral delivery, are able to deliver the ABE mRNAs and gRNA to the target tissues (Newby and Liu, Mol Ther 29:3107–3124, 2021). This chapter mainly introduces the production and LNP delivery of ABE mRNA and gRNA.

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Maximum Likelihood Hebbian Learningools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.

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