Titlebook: Base Editors; Methods and Protocol Sangsu Bae,Beomjong Song Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

concession

书目名称Base Editors影响因子(影响力)




书目名称Base Editors影响因子(影响力)学科排名




书目名称Base Editors网络公开度




书目名称Base Editors网络公开度学科排名




书目名称Base Editors被引频次




书目名称Base Editors被引频次学科排名




书目名称Base Editors年度引用




书目名称Base Editors年度引用学科排名




书目名称Base Editors读者反馈




书目名称Base Editors读者反馈学科排名

嬉耍

打折

Delivering Base Editors In Vivo by Adeno-Associated Virus Vectorstissues. We provide step by step protocols for (i) designing and validating base editing systems, (ii) packaging base editors into recombinant AAV vector particles, (iii) delivering AAV to the central nervous system via intrathecal injection, and (iv) quantifying base editing frequencies by next-generation sequencing.

Estimable

Anthropoid

Maximum Likelihood Hebbian Learningning-based computational models to predict the efficiencies and outcome frequencies of ABE and CBE at given target DNA sites, in silico. Here, we describe the step-by-step procedure for the accurate determination of specific target nucleotides for ABE or CBE editing on the online available web tool, (DeepBaseEditor, .).

啜泣

容易懂得

anthropologist

使服水土

Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seqving uracil in vitro. Digested genomic DNA is subjected to WGS, and then sequencing reads are aligned to the reference genome, resulting in straight alignments at on-target and off-target sites. The in vitro cleavage sites related to the straight alignments can be identified using the Digenome-seq computer tool.

窒息

Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors introduced the target nucleotide substitution in living cells and identified variants whose functions were not defined. Here, we describe the methods for the functional appraisal of BRCA1 variants using CRISPR-based base editors.