Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2016Latest edition Springer Science+Business Media, LLC 2016 BEV

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https://doi.org/10.1007/978-3-531-90142-8 by the bioreactor unit. The Cellbag can be filled with medium and cells and maintained at a set temperature. The rocking motion, which is adjusted through angle and rock speed settings, provides mixing of oxygen (and CO., which is used to control pH in mammalian cell cultures) from the headspace cr

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Recombinant Baculovirus Isolationge in which insect cells are transfected with either the virus genome alone (Bac-to-Bac. or BaculoDirect™, Invitrogen) or virus genome and transfer vector. In the latter case, this allows the natural process of homologous recombination to transfer the foreign gene, under control of the polyhedrin or

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Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculovirusesare presented that are appropriate for different cell lines dependent upon the growth characteristics (in particular, cell attachment properties) of the cells of interest. In addition to the routine maintenance of cells in active culture, methods are also described for both short-term (low temperatu

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