Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2016Latest edition Springer Science+Business Media, LLC 2016 BEV

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Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors by the bioreactor unit. The Cellbag can be filled with medium and cells and maintained at a set temperature. The rocking motion, which is adjusted through angle and rock speed settings, provides mixing of oxygen (and CO., which is used to control pH in mammalian cell cultures) from the headspace cr

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Laura Marie Wingender,Hans-Georg Wolff the isolation of recombinant baculoviruses (Chapter . provides a full account of the different systems and transfer vectors available). The practical details cover: transfection of insect cells with either virus DNA or virus DNA and plasmid transfer vector; a reliable plaque-assay method that can b

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