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Titlebook: Baculovirus Expression Protocols; Christopher D. Richardson Book 19951st edition Humana Press 1995

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Una M. Röhr-Sendlmeier,Udo Käser the foreign gene has been correctly integrated. Having proven the presence of the gene, cells are infected with several independent recombinant viruses, in order to determine which of those gives the best expression of the protein in insect cells.
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Innovationen in der Gesundheitswirtschaftnner flask cultures cannot provide ample material. Depending on the quantity required, bioprocesses can vary in complexity from short-term (5-7 d) batch cultures to longer-term fed-batch (7-10 d) or perfusion processes (7 to 2 30 d).
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Production of recombinant baculoviruses using linearized viral DNA,ad of the native viral DNA, which is circular, the proportion of progeny viruses that are recombinant increases to about 25% (.). This can greatly reduce the work involved in screening for recombinant viruses.
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