Titlebook: Baculovirus Expression Protocols; Christopher D. Richardson Book 19951st edition Humana Press 1995

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书目名称Baculovirus Expression Protocols影响因子(影响力)




书目名称Baculovirus Expression Protocols影响因子(影响力)学科排名




书目名称Baculovirus Expression Protocols网络公开度




书目名称Baculovirus Expression Protocols网络公开度学科排名




书目名称Baculovirus Expression Protocols被引频次




书目名称Baculovirus Expression Protocols被引频次学科排名




书目名称Baculovirus Expression Protocols年度引用




书目名称Baculovirus Expression Protocols年度引用学科排名




书目名称Baculovirus Expression Protocols读者反馈




书目名称Baculovirus Expression Protocols读者反馈学科排名

Allowance

Preserve

Insect Cell Culture in Serum- Free Media,ntial replication of entomopathogenic viruses in vitro, there have been over 400 cell lines established to date from various insect species (.). Without exception, the cell lines described in the literature and currently in use have been isolated and established from primary cultures using serum-sup

Criteria

Transfection Techniques for Producing Recombinant Baculoviruses,es for foreign gene expression. A variety of transfection methods, which were first developed for mammalian cell lines, have been adapted for use with insect tissue-culture cells. Conditions required for the maximum transfection efficiency of any vertebrate or invertebrate cell line vary considerabl

resuscitation

Selection of Recombinant Baculoviruses by Visual Screening,on of recombinants is rapid compared to mammalian expression systems, whereas the capacity to perform many posttranslational modifications necessary for the expression of authentic, functional proteins is retained. Examples of glycosylation (.), phosphorylation (.), myristilation (.), palmitoylation

极大痛苦

Production of recombinant baculoviruses using linearized viral DNA,enome with the corresponding segment from a transfer vector that has been modified to include a foreign gene. The frequency of this recombination is low; most of the input viral DNA molecules do not recombine with a plasmid DNA. Typically, only 0.1-2% of the progeny viruses are recombinant; hence, l

Allodynia

消耗

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