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Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski Book 2009 Humana Press 2009 DNA.Laboratory.Microarray.PCR.Polya

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Dieter Steegmüller,Michael ZürnBasic Local Alignment Search Tool (BLASTX) to identify genes through homologs, a variety of tools are discussed by their creators. These include for genome annotation: GeneMark, Artemis, and BASys; and, for genome comparisons: Artemis Comparison Tool (ACT), Mauve, CoreGenes, and GeneOrder.
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Plug&Play-Fördertechnik in der Industrie 4.0ocuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.
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Characterization of DNA Motifs with Particular Reference to Promoters and Terminatorsand resources to identify regulatory elements are listed and discussed. Combining the available web-resources and literature data optimizes these predictions and can thus aid in a more directed experimental identification of these regulatory elements.
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PCR and Partial Sequencing of Bacteriophage Genomesetween phages which infect fairly divergent taxa. As more sequence data become available through both metagenomic approaches and the sequencing of complete bacteriophage genomes, PCR primers can be further refined and thus it should be an increasingly useful tool for bacteriophage biology.
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Michael ten Hompel,Michael Henkeacteriophage DNA from one or two 9 cm L-agar plates. For these preps, the use of agarose in the top agar is recommended to avoid any restriction inhibitors that may be present in some agar preparations.
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