Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski Book 2009 Humana Press 2009 DNA.Laboratory.Microarray.PCR.Polya

吵闹

Scintigraphy

就职

Feckless

Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinttify the diversity of natural viral communities. Currently, two different methodological approaches are widely used to obtain genetic fingerprints of natural phage communities. While the separation of different viral genomes with pulsed field gel electrophoresis (PFGE) is based on the size of the ge

Small-Intestine

Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenomethin the universally conserved gene for small subunit ribosomal RNA (16S rDNA). A plethora of methodologies for characterizing the diversity and composition of bacterial communities is based on sequence polymorphisms within this single gene. By comparison, no gene is universally shared among viruses

专横

Armin Lechler,Jan Schlechtendahletween phages which infect fairly divergent taxa. As more sequence data become available through both metagenomic approaches and the sequencing of complete bacteriophage genomes, PCR primers can be further refined and thus it should be an increasingly useful tool for bacteriophage biology.

呼吸

Stefan Feldmann,Birgit Vogel-Heuserion and quantification of high quality RNA from infected bacterial cells, using the marine cyanobacterium WH7803 and the phage S-PM2 as a case study. This protocol can be modified to extract RNA from the host/bacteriophage of interest.

僵硬

Kay Fürstenberg,Christopher Kirscho how probe length, secondary structure, free energy, probe orientation and amplification all have to be taken into account. As means of an example, the conditions used for the hybridisation of an array designed to be specific to the cyanophage S-PM2 is detailed.

stress-test

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