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Titlebook: Bacterial and Archaeal Motility; Tohru Minamino,Makoto Miyata,Keiichi Namba Book 2023 The Editor(s) (if applicable) and The Author(s), und

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Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagelmbrane stator complex over-expressed in . cells using fluorescent indicators. Intensity measurements of fluorescent indicators using either a fluorescence spectrophotometer or microscope allow quantitative detection of changes in the intracellular ion concentrations due to the ion channel activity of the transmembrane protein complex.
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Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopyhe binding of Na. to purified PomAB stator complex using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. This method demonstrates that Na.-binding sites are formed by critical aspartic acid and threonine residues located in the transmembrane segments of PomAB.
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Allgemeiner Rahmen des Vergaberechtsa wide range of external load, but the elasticity of the axial parts of the flagellum, such as the hook and filament, limits the spatiotemporal resolution. In this chapter, we describe a bead assay optimized for the analysis of the flagellar motor dynamics at near zero load.
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