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Titlebook: Bacterial and Archaeal Motility; Tohru Minamino,Makoto Miyata,Keiichi Namba Book 2023 The Editor(s) (if applicable) and The Author(s), und

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Analysis of Adhesion and Surface Motility of a Spirochete Bacteriumamphibious motility is believed to be significant for pathogenicity. This chapter focuses on the zoonotic spirochete . and describes the method for measuring the spirochete adhesion and surface motility.
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Purification of the Transmembrane Polypeptide Channel Complex of the , Flagellar Type III Secretion he export gate complex, and this core complex serves as a polypeptide channel that allows flagellar structural subunits to be translocated across the cytoplasmic membrane. Here, we describe the methods for overproduction, solubilization, and purification of the . FliP/FliQ/FliR complex.
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Swarming Motility Assays in daptive resistance to antibiotics. Described here are protocols to cultivate, verify, and study swimming and swarming motility in ., and an additional “border-crossing” assay, where cells “primed” to swarm are presented with an environmental challenge such as antibiotics to assess their propensity to handle the challenge.
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Force Measurement of Bacterial Swimming Using Optical Tweezers of bacteria could develop problems with the accuracy of the force estimation. This chapter describes the force measurement using optical tweezers. The method uses parameters obtained from the shape and movement of a microsphere attached to the bacteria, improving the quantitativeness of force measurement.
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