Titlebook: Bacterial Transcriptional Control; Methods and Protocol Irina Artsimovitch,Thomas J. Santangelo Book 2015 Springer Science+Business Media N

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https://doi.org/10.1007/978-3-8348-9245-4 polymerases are currently elucidated by structural, genetic, and biochemical approaches. Here, we describe a fast and reliable approach to quantitative analyses of transcription fidelity, applicable to analyses of RNA polymerase selectivity against misincorporation, incorporation of dNMPs, and chem

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https://doi.org/10.1007/978-3-8348-9245-4uct, nucleotide substrates, metal cofactors, and regulatory molecules that bind to distinct RNAP sites to modulate its activity. RNAP is also inhibited by several known antibiotics and is a promising target for development of novel antibacterial compounds. Despite great progress in structural analys

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https://doi.org/10.1007/978-3-8348-9245-4mpared to its pol II counterpart, Pol III has several advantages, including the relative simplicity, stability, and more direct connectivity of its transcription machinery. Only two transcription factor complexes, TFIIIB and TFIIIC, are required to faithfully initiate and direct multiple rounds of t

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Wilfried Plaßmann,Detlef Schulzlymerase–promoter complex proceeds through multiple intermediates. Short promoter fragments can be used as a tool to dissect RNA polymerase–promoter interactions and to pinpoint elements responsible for specific properties of the entire promoter complex. A recently developed fluorometric molecular b

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https://doi.org/10.1007/978-3-8348-9245-4RNA (5′ specific RNA-seq). The protocol describes how cDNA libraries for 5′ specific RNA-seq can be tailored to analyze specific classes of RNAs based upon the phosphorylation status of the 5′ end. Thus, the analysis of cDNA libraries generated by these methods provides information regarding both th

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https://doi.org/10.1007/978-3-8348-9245-4n include pausing, backtracking, arrest, and transcription termination, and it is critical to determine whether the absence of continued synthesis is transient or permanent. Here we describe mechanisms to generate large quantities of stable archaeal elongation complexes on a solid support to permit