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Titlebook: Bacterial Transcriptional Control; Methods and Protocol Irina Artsimovitch,Thomas J. Santangelo Book 2015 Springer Science+Business Media N

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发表于 2025-3-21 16:42:25 | 显示全部楼层 |阅读模式
期刊全称Bacterial Transcriptional Control
期刊简称Methods and Protocol
影响因子2023Irina Artsimovitch,Thomas J. Santangelo
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Transcriptional Control; Methods and Protocol Irina Artsimovitch,Thomas J. Santangelo Book 2015 Springer Science+Business Media N
影响因子.This volume. .is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of .in vitro .and .in vivo. technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful .Methods of Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. .Practical and timely, .Bacterial Transcriptional Controls: Methods and Protocols. highlights the breadth
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Purification of Bacterial RNA Polymerase: Tools and Protocols, the use of multicistronic vectors for expression of the . enzyme in large quantities and in a highly active form. Here we describe the commonly used vectors and procedures for purification of the . RNA polymerase.
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Differential- und Integralrechnung the use of multicistronic vectors for expression of the . enzyme in large quantities and in a highly active form. Here we describe the commonly used vectors and procedures for purification of the . RNA polymerase.
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In Situ Footprinting of , Transcription Elongation Complex with Chloroacetaldehyde,cetaldehyde for in situ footprinting of . elongation complex temporarily halted by a protein roadblock. The method provides valuable information on the dynamic features of transcriptionally engaged RNA polymerase within the cellular environment.
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Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initnding assays from series used to determine the urea dependence of open complex formation and dissociation with . RNA polymerase and phage λP. promoter DNA. Then, we describe the subsequent data analysis and interpretation of these solute effects.
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