Titlebook: Bacterial Toxins; Methods and Protocol Otto Holst Book 2000 Springer Science+Business Media New York 2000

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Purification of , Type A Neurotoxin, animals (.). In recent years, injection of crystalline botulinum toxin type A has been demonstrated to provide relief from certain involuntary muscle disorders, dystonic conditions, pain syndromes, and headaches (.,.,.). The use of botulinum toxin for treatment of human disease and its usefulness i

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Shiga Toxins, ago. Up until the early 1980s this group of toxins was little more than a scientific curiosity without a clear role in disease pathogenesis. However, since the discovery of these toxins in . and other Enterobacteriaceae, and their association with identifiable clinical diseases such as hemorrhagic

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Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins,ver toxins, microcystins. Closely related toxic cyclic pent ape ptides, nodularins, have been isolated from the brackish water cyanobacterium .. In addition, some cyanobacteria produce neurotoxic compounds such as anatoxin-a, anatoxin-a(s), and saxitoxins. Recent reviews of the chemical and biologic

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Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins,he precise mapping of the molecular determinants of their activity. The results of such studies are of particular importance for the elucidation of the mode of action of the toxins and the design of genetically engineered molecules displaying novel activity. For example, a large effort is currently

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Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylocey include α-toxin (or α-hemolysin), which is probably one of the best studied toxins (.), and a large group of bicomponent toxins formed by the leukocidins and the Γ-hemolysins (.). All these toxins share sequence homology (.) as well as functional similarity (.) which altogether suggest they proba

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The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxinf two competing processes. In radiative de-excitation, a brief relaxation time (about 10. s) is followed by the electron’s return to the ground state accompanied by the emission of a photon whose wavelength is longer than the one absorbed (.). Fluorescence emission competes with nonradiative process

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