Titlebook: Bacterial Persistence; Methods and Protocol Jan Michiels,Maarten Fauvart Book 2016 Springer Science+Business Media New York 2016 Antibiotic

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https://doi.org/10.1007/978-3-531-92520-2tions of currently used antifungals. In this chapter, the method to determine the persister fraction in a . biofilm treated with an antifungal compound is described. To this end, a mature biofilm is developed and subsequently treated with a concentration series of the antifungal compound of interest

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Stefan Hummelsheim,Dieter Timmermanninfections, and therefore, understanding their physiology may lead to novel therapeutics to treat recalcitrant infections. However, persisters have yet to be isolated due to their low abundance, transient nature, and similarity to the more highly abundant viable but non-culturable cells (VBNCs), res

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Wilfried Bos,T. Neville Postlethwaitefabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

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Psychologische Bildungsforschungd persister cell formation. Research in this field has mainly focused on the model organism . and few genetic determinants of persistence have been described in other bacterial species, impairing the development of target-based strategies to combat these antibiotic-tolerant cells. In this chapter we

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Wilfried Bos,T. Neville Postlethwaitebinant TA genes into inducible plasmids and expressing these in . Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in . and whether expression can affect persister cell formation.