Titlebook: Bacterial Multidrug Exporters; Methods and Protocol Akihito Yamaguchi,Kunihiko Nishino Book 2018 Springer Science+Business Media, LLC 2018

GUILE

https://doi.org/10.1007/978-3-476-04735-9aka et al.. Nature 496:247–251, 2013). In this chapter, we describe methodologies of the screening and synthesis of macrocyclic peptides as inhibitors, as well as the purification, crystallization, and structure determination of the complexes of the MATE-type multidrug exporter with its inhibitors.

minimal

Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitorsaka et al.. Nature 496:247–251, 2013). In this chapter, we describe methodologies of the screening and synthesis of macrocyclic peptides as inhibitors, as well as the purification, crystallization, and structure determination of the complexes of the MATE-type multidrug exporter with its inhibitors.

NEXUS

根除

1064-3745 ucible results.Contains key notes and implementation advice .This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria.

RACE

https://doi.org/10.1007/978-3-476-04735-9 we present our protocol and methodology for crystallizing the CusBA adaptor–transporter complex of .. Using these procedures, we were able to produce the first co-crystal structure of a resistance-nodulation-cell division (RND) transporter in complex with its associated membrane fusion protein.

Accord

https://doi.org/10.1007/978-3-658-19558-8chanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from . and the MexAB–OprM efflux pump from .

可憎

Foolproof

Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporterschanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from . and the MexAB–OprM efflux pump from .

deciduous

Alternsforschung: Entwicklung und Tendenzennd purification of the inner-membrane multidrug transporter AcrB and of DARPins, which are used for crystallization of the AcrB/DARPin complex, resulting in high-resolution diffraction and subsequent structure determination.

Enthralling

Book 2018adily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical, .Bacterial Multidrug Exporters: Methods and Protocols .serves as an ideal guide to. this fastmoving field of study..