Titlebook: Bacterial Artificial Chromosomes; Volume 2: Functional Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004

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Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes,nsively comparing these applications. Although the basic technique for microinjection of large constructs are similar to those used for shorter DNA segments, there are some special requirements (.). In this chapter, we describe relevant steps for microinjection using large DNA constructs.

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Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors,tial for HSV-1 replication can only be produced on complementing cell lines (.). A more-efficient strategy for mutating HSV-1 was achieved by cloning the entire virus genome as a set of overlapping clones in cosmids. This allows the manipulation of specific virus genes on individual cosmid clones in

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https://doi.org/10.1007/978-3-540-79567-4 the number of CpG islands in the human genome was estimated to be 34,200 (4 as modified by 7) and this figure is reasonably close to the 28,890 potential CpG islands that have been identified so far in the draft human genomic sequence (.).

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Kathija Yassim,Neville Rudman,Lucky Maluleke to ensure the stability of the insert. However, this hinders manipulation of BACs by homologous recombination in the bacterial cells. Consequently, the gene(s) required for recombination is transiently expressed to facilitate homologous recombination. Several different systems have been developed f

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https://doi.org/10.1007/978-3-319-08825-9nsively comparing these applications. Although the basic technique for microinjection of large constructs are similar to those used for shorter DNA segments, there are some special requirements (.). In this chapter, we describe relevant steps for microinjection using large DNA constructs.