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Titlebook: Bacterial Artificial Chromosomes; Volume 2: Functional Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004

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发表于 2025-3-21 20:05:11 | 显示全部楼层 |阅读模式
期刊全称Bacterial Artificial Chromosomes
期刊简称Volume 2: Functional
影响因子2023Shaying Zhao,Marvin Stodolsky
视频video
发行地址Includes supplementary material:
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Artificial Chromosomes; Volume 2: Functional Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004
影响因子Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so
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Exon Trapping for Positional Cloning and Fingerprinting,ies, or phenotypes, to a quantitative trait locus (QTL), a genomic region no smaller than 1 centiMorgan (cM) or megabase (Mb) in length. Physical mapping can then provide a map of higher resolution. Physical maps are constructed from clones identified by screening genomic libraries. Genomic clones c
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Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column,ize, which are free of methylation and these are known as CpG islands (. . . and . for reviews). In addition to distinctive DNA characteristics, CpG islands have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (.). The major reason for
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BAC Microarray-Based Comparative Genomic Hybridization,ridization (array CGH) provides a high throughput means to measure and map copy number aberrations on a genome-wide scale and to link them directly to genome sequence. For applications involving the analysis of tumors, the technology must provide reliable detection of single copy gains and losses in
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Large DNA Transformation in Plants,). Both methods used modified bacterial artificial chromosomes (BACs) such as BIBAC or pBACwich. BIBAC vector (used in tomato) is capable of transferring large DNA fragments from . into plants. pBACwich (used in tobacco) utilizes a Cre/. site-specific recombination system to integrate large DNA frag
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BAC Modification Using a RecA Expressing Shuttle Vector System,BACs have the capacity for maintaining large, often more than 300 kb fragments of mammalian DNA as stable inserts and avoid much of the insert chimerism which characterizes yeast artificial chromosomes (YACs). Isolation of BAC DNA is easy because they exist as supercoiled circular plasmids that are
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BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes,b), which can accommodate most eukaryotic genes along with their full set of regulatory elements, and to their greater convenience in handling over other large cloning vectors like P1-based artificial chromosomes (PACs) and yeast artificial chromosomes (YACs). Two key advances for harnessing the ful
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