Titlebook: Autophagy; Methods and Protocol Nicholas Ktistakis,Oliver Florey Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

RAG

angina-pectoris

欢乐中国

An Ocean Bottom Absolute Gravity Meter identifying putative LIR motifs in a whole protein sequence using peptide arrays generated by SPOT synthesis on nitrocellulose membranes. The use of two-dimensional peptide arrays allows for further identification of specific residues critical for LIR binding.

时代错误

Curvilinear Coordinates and General Tensors,and isolation membrane-associated tubules (IMATs). These methods can be easily applied to cultured mammalian cells for conventional and cutting-edge EM analyses, leading to a better understanding of ultrastructural details in autophagosome formation.

灰姑娘

泥土谦卑

Recombinant Expression, Purification, and Assembly of p62 Filaments, as well as the preparation of negative stain EM grids to visualize the filaments. In vitro formed p62 filaments can be used to study receptor cargo interactions in minimal reconstituted autophagy model systems.

绊住

Biophysical Studies of LC3 Family Proteinsvesicles, (b) ultracentrifugation and fluorescence methods for assaying protein binding to membranes, and (c) procedures for assessing vesicle–vesicle aggregation and fusion. The latter include methods for intervesicular total lipid mixing, mixing of lipids in the vesicle inner monolayers, and aqueous contents mixing.

易受刺激

组成

Improved Electron Microscopy Fixation Methods for Tracking Autophagy-Associated Membranes in Cultureand isolation membrane-associated tubules (IMATs). These methods can be easily applied to cultured mammalian cells for conventional and cutting-edge EM analyses, leading to a better understanding of ultrastructural details in autophagosome formation.

潜伏期

Methods for Imaging Autophagosome Dynamics in Primary Neuronsority of autophagosomes in dendrites are stationary, while some exhibit bidirectional movement. These studies establish an initial road map for autophagosome dynamics in each compartment of the neuron and set the stage for a more detailed understanding of neuronal autophagy in stress and disease.