Titlebook: Autophagy; Methods and Protocol Nicholas Ktistakis,Oliver Florey Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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G. Boedecker,I. Marson,H.-G. Wenzelrecursors and report that the formation of autophagosomes is associated with a strong relaxation of membrane curvature energy. This effect we find for a wide range of membrane asymmetries. It is especially strong for small distances between both autophagosomal membranes, as observed in vivo. We quan

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,Experimental Tests of Einstein’s Theory, imaging-based autophagy assessment methodologies can be applied to these neurons, thereby providing a detailed account of the application of imaging-based autophagy assays to human iPSC-derived mDANs.

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1064-3745 ation advice from the experts.This volume details a comprehensive and extensive set of protocols for the study of autophagy .in vitro. and .in vivo.. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autoph

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,Einstein’s Law of Gravitation,gosomes. To do so, we set up three cameras on one microscope to be able to acquire three colors at the same time. Here we describe the setup using a Yokogawa spinning disk confocal microscope (CSU-W1) with Andor TuCam system attaching 3 × Zyla 4.2 CMOS cameras (Andor) and detail the method for acquiring live images.

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Structural Studies of Autophagy-Related Proteinsproteins involved in autophagy by using X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy. Further we will provide instructions how to perform a molecular dynamics (MD) simulation using GABARAP as example protein.

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Cell-Free Reconstitution of Autophagic Membrane Formationmbrane interactions underlying autophagic membrane precursor generation have been a focus of attention but yet poorly defined. Here, we summarize the procedure of a cell-free system we have established to dissect the molecular mechanism of early autophagic membrane generation.