Titlebook: Auditory and Vestibular Research; Methods and Protocol Bernd Sokolowski Book 2009 Humana Press 2009 cells.gene expression.gene transfer.gen

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In the Beginning was Game Semantics?his chapter provides a detailed description and critical evaluation of the methodology used to transfect cDNA expression constructs, including green fluorescent protein (GFP) tagged full-length cDNAs of myosin XVa, whirlin, and β-actin, into cultured inner ear sensory epithelia using the Bio-Rad Helios® Gene Gun.

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Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epitheliumhis chapter provides a detailed description and critical evaluation of the methodology used to transfect cDNA expression constructs, including green fluorescent protein (GFP) tagged full-length cDNAs of myosin XVa, whirlin, and β-actin, into cultured inner ear sensory epithelia using the Bio-Rad Helios® Gene Gun.

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Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Earsented. Expression plasmid encoding a gene of interest is microinjected through the uterine wall into the lumen of the otocyst and electroporated into otic epithelial progenitor cells. Downstream analysis of the transfected embryonic or postnatal inner ear is then conducted to gain insight into gene function.

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1064-3745 croscopic imaging.Clearly describes the protocols necessary Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality of sign language, cognitive and language development c

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Wildlife Overpopulation Controlcharacterization of isolates’ genetic relatedness, yet they differ in the types of studies for which they are most useful. Consequently, knowledge of both techniques is important for laboratories intending to study the molecular epidemiology of otitis media–associated bacterial pathogens.

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Gamete/Embryo-Fetal Origins of Tumours,shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry.

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