Titlebook: Atherosclerosis; Experimental Methods Angela F. Drew Book 2001 Humana Press 2001

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Culture of Human Endothelial Cells,l0. cells and covers an area of 1-7 m.. Endothelium serves many functions, including fluid and solute exchange through cell contraction, provision of an antithrombogenic surface through tissue plasminogen activator (tPA) and prostacyclin release, synthesis of angiogenic factors such as adenosine, al

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Identification of Cell Types and Quantification of Lesion Composition,ed macrophages, lymphocytes, and mast cells. Examining the role each cell type plays in the formation and pathophysiology of atherosclerotic plaques relies upon the identification of these cells within plaques and early plaque-like structures, which in turn depends on their expression of recognizabl

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Evaluation of Angiogenesis,urgical treatment, and thus amputation is required. A substantial portion of these amputations might be avoided by stimulating blood vessel growth to the affected lower extremity. In a new therapeutic approach, certain recombinant growth factors, administered as genes or recombinant proteins, have b

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9 Assay for Serum Glycated Lipoproteins, LDL that exists in the circulation is glycated LDL. It has been shown to be more easily oxidized than native LDL (.-.). These findings indicate that the glycation of LDL may be the initial step in the development of atherosclerosis.