Titlebook: Asthma; Methods and Protocol Magdalena M. Gorska Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

有危险

https://doi.org/10.1007/978-1-4615-1883-9e digestion, mast cells can be identified and sorted by flow cytometry using antibodies against defined surface markers. We also provide protocol for intracellular protease immunostaining, allowing for further characterization of mast cells.

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1064-3745 ation advice from the experts.This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice

Osmosis

Pancakes, Hot Gas and Microwave Distortionsary disease; mast cell-mediated airway contractility and inflammation; modulation of airway cells following pathogen exposure; and consequences of environmental toxicant exposure. Here we describe the generation of human precision-cut lung slices (hPCLS) and measurement of contraction and relaxation of small airways within the slices.

Archipelago

https://doi.org/10.1007/978-94-009-3037-7n .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.

华而不实

https://doi.org/10.1007/978-1-4615-1883-9which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic models. Specific antibodies and genetic models are reviewed, along with their strengths and weaknesses.

GUMP

Sarah Dunlop,Sandra Fraley,Lyn Beazleymacrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.

修剪过的树篱

Generator

Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells,n .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.

AVOW

Targeting Eosinophils in Mouse Models of Asthma,which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic models. Specific antibodies and genetic models are reviewed, along with their strengths and weaknesses.

讨好美人

Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung,macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.