Titlebook: Asthma; Methods and Protocol Magdalena M. Gorska Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Isolation, Ex Vivo Culture, and Stimulation of Tracheal and Nasal Chemosensory Cells,in the airway epithelium and are equipped with the machinery to generate prostaglandins and acetylcholine. Activation of innate type 2 lymphoid cells and dendritic cells triggered by brush cell-derived mediators skew the immune response in the airway to type 2 inflammation that underlies atopic dise

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Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells,2s, our method utilizes the surface markers CD127 and ST2 to classify a group of type 2 cytokine-producing ILC2s upon activation by the fungal allergen .. Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sor

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Detection of Eosinophils in Tissue Sections by Immunohistochemistry,minantly reside in certain organs, such as the gastrointestinal tract. However, eosinophil numbers increase both in the peripheral blood and tissues during allergic inflammation, parasitic infestation, drug reactions, vasculitides, as well as certain hematopoietic neoplasms. Their presence in tissue

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Targeting Eosinophils in Mouse Models of Asthma,sthma and models of allergic airway inflammation. Their role in the pathophysiology of asthma and other diseases can be studied using mouse models in which eosinophils are depleted. This review article focuses on two main approaches for depleting eosinophils in vivo, antibody-mediated and genetic mo

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Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human, inflammation. While mast cell progenitors can be found in peripheral blood, phenotypically mature mast cells can only be found within peripheral tissues. Here, we describe optimized tissue digestion protocols for obtaining mast cells from the murine lung and from human sinus tissue. Following tissu

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Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung, homeostasis. During inflammation, these are joined by monocyte-derived recruited macrophages. Flow cytometry can be used to identify and purify lung macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mou

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Mass Cytometry Analysis of Whole Blood Response to an Allergen,An advantage over fluorescence cytometry is the relative lack of background, which provides excellent resolution for detection of phosphoproteins and quantification of cell signaling. We have applied mass cytometry to the analysis of whole blood staining after ex vivo stimulation with peanut allerge

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