Titlebook: Arthritis Research; Methods and Protocol Shunichi Shiozawa Book 2014Latest edition Springer Science+Business Media New York 2014 autoimmune

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Induction of , Autoimmune Disease in Normal Mice upon Repeated Immunization with Antigen, induce disease in normal mice not having a genetic disposition to autoimmunity. We describe here a novel and completely reproducible experimental technique that can induce systemic autoimmunity or systemic lupus erythematosus (SLE) in mice otherwise not prone to spontaneous autoimmune disease. This

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Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iug treatment, are urgently needed to select adequate drugs for patients. Gene mutation status, protein expression levels in a biopsy, and serum proteins are often used as biomarkers. One of the methods to screen for protein biomarkers involves quantitative proteomic approaches using mass spectrometr

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Genome-Wide Genetic Study in Autoimmune Disease-Prone Mice,g on their abnormal quantitative phenotypes in relation to the loss of self-tolerance will lead to the identification of polymorphic genes that play pivotal roles in the genetic predisposition to autoimmunity. In this chapter, we first overview the basic concepts in the statistical genetics and then

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Sample Processing, Protocol, and Statistical Analysis of the Time-of-Flight Secondary Ion Mass Speced to distinguish healthy and osteoarthritic human cartilage. This chapter discusses ToF-SIMS principle and instrumentation including the three modes of analysis in ToF-SIMS. ToF-SIMS sets certain requirements for the samples to be analyzed; for example, the samples have to be vacuum compatible. Acc

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Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy,al stromal cells (MSCs) offer an attractive and convenient alternative to conventional fixative based lipid dyes such as Oil Red O and Sudan Red, fluorescent labels such as LipidTOX, and more indirect methods such as qRT-PCR analyses of specific adipocyte differentiation markers such as peroxisome P

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Book 2014Latest edition arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods f