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Titlebook: Arthritis Research; Volume 1: Methods an Andrew P. Cope Book 2007 Humana Press 2007 Arthritis.PCR.gene expression.gene transfer.genes.hybri

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Assessment of Gelatinase Expression and Activity in Articular Cartilagealysis of tissue sections provides information about the precise localization of the enzymes within the tissue, pinpointing the cells that synthesize the proteinases, and zymography of cell/tissue conditioned culture media allows a semi-quantitative assessment of the gelatinases and their activation
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Analyses of MT1-MMP Activity in Cellsthe characteristic functions of this enzyme is activation of proMMP-2 on the cell surface (.). This process can be monitored using gelatin zymography to detect the pro- and active forms of MMP-2. Cellular MT1-MMP activity can also be directly detected by . degradation of layers of either gelatin or
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Andrew P. CopeBroad range of topics covering many aspects of rheumatic disease.In-depth studies of cutting-edge technology.Robust tools for translational research.Contributions from leaders in their fields
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Methods in Molecular Medicinehttp://image.papertrans.cn/b/image/161918.jpg
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Measurement of Glycosaminoglycan Release from Cartilage Explantscy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method
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