Titlebook: Arthritis Research; Volume 1: Methods an Andrew P. Cope Book 2007 Humana Press 2007 Arthritis.PCR.gene expression.gene transfer.genes.hybri

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书目名称Arthritis Research影响因子(影响力)




书目名称Arthritis Research影响因子(影响力)学科排名




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书目名称Arthritis Research网络公开度学科排名




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书目名称Arthritis Research被引频次学科排名




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书目名称Arthritis Research读者反馈学科排名

Geyser

Hybridization of Synovial Tissuee, specific, and sensitive, because even small quantities of mRNA can be detected. Presented here contemporary protocols for ISH using a combined nonradioactive immunohistochemical detection system..In overview, the following steps have to be covered to perform ISH. (1) mRNA probes (sense and antise

存心

Subtractive Hybridization is a screening method for differentially expressed genes. At first, poly-A.-RNA is isolated and reverse transcribed into cDNA. With the SMART technology, total RNA can be used. For subtractive hybridization, two adaptors are ligated to the tester cDNA. The tester cDNA is then mixed twice with cDNA

ironic

Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synoviumal biopsies. However, this strategy does not distinguish between specific gene expression profiles of cells originating from discrete tissue areas. Therefore, we established the combination of laser-mediated microdissection and RNA arbitrarily primed polymerase chain reaction (RAP-PCR) for different

Hypopnea

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粗鲁的人

进取心

Image Analysis of Aggrecan Degradation in Articular Cartilage With Formalin-Fixed Samplesrtilage. The early histochemical/histological scale of Mankin (.) has been widely used but recently challenged as insufficient (.). Imaging techniques such as microscopic magnetic resonance imaging (MRI) (.), polarized light microscopy (.), atomic force microscopy (.), and infrared spectral analysis

SOW

影响

Measurement of Glycosaminoglycan Release from Cartilage Explantscy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method