Titlebook: Archaea; Methods and Protocol Sébastien Ferreira-Cerca Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

FAWN

sulcus

Book 2022 and step-by-step, readily reproducible protocols..Authoritative and cutting-edge, .Archaea: Methods and Protocols. aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field..

裤子

Book 2022 access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic

木讷

Current Topics in Veterinary Medicineroduction of the . gene on a plasmid leads to a selectable loss of 8-ADP resistance after a homologous recombination event (pop-in). A subsequent pop-out event restores the 8-ADP resistance and can generate chromosomal mutants with frequencies of about 50%.

Modicum

Patricia A. Barton-Gade,Eli V. Olsenales. In this chapter, we describe the methods for the available genetic tools in . including a (1) transformation method, (2) pop in/pop out strategy to generate markerless deletion mutants and (3) a plasmid-based expression system.

GUILT

Multi-Dimensional Preference Models,that allowed the successful analysis of relative protein synthesis and degradation rates on the proteome scale of the halophilic archaeon .. This method combines .N and .C isotope metabolic labeling with high-resolution mass spectrometry and data analysis tools (QuPE web-based platform) and could be applied to different archaea.

paragon

HARP

Assessment for conservation and protection,n into the genome or through retention of autonomously replicating plasmids. The robust genetic system developed in . offers a variety of selectable and counterselectable markers for complex, accurate and iterative genetic manipulations offering greater flexibility to probe gene function in vivo.

LATHE

https://doi.org/10.1007/0-387-31099-1s of only a few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries using size-selected total RNA in the model archaeon, ., to provide a tool to further characterize sRNAs in archaea.

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