Titlebook: Archaea; Methods and Protocol Sébastien Ferreira-Cerca Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

整顿

COW

Hippocampus

pulmonary-edema

Archaea978-1-0716-2445-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

BILE

John F. Stevenson,John C. Norcrosslable model archaeal genetic systems. We consider the limitations and barriers involved in genetically modifying different archaeal species, the techniques and breakthroughs that have contributed to their tractability, and potential areas for future development.

sparse

光亮

https://doi.org/10.1007/978-1-4615-1593-7rtion mutant library in this crenarchaeal model. We envision that the traditional transposon-based forward mutagenesis screening paired with next generation sequencing will greatly speed up the exploration of archaeal genomic features.

Ornithologist

https://doi.org/10.1007/0-387-31099-1ng library from culture harvesting, footprint isolation via ultracentrifugation, gel-based size fractionation, and footprint sequencing for a model halophilic archaeon, .. This approach has revealed the first global view of translation in the archaea.

大笑

Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon rtion mutant library in this crenarchaeal model. We envision that the traditional transposon-based forward mutagenesis screening paired with next generation sequencing will greatly speed up the exploration of archaeal genomic features.

minimal

Conversion of Ribosome-Protected mRNA to DNA and Deep Sequencing for Ribosome Profiling in ng library from culture harvesting, footprint isolation via ultracentrifugation, gel-based size fractionation, and footprint sequencing for a model halophilic archaeon, .. This approach has revealed the first global view of translation in the archaea.