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Titlebook: Applications of Mass Spectrometry in Microbiology; From Strain Characte Plamen Demirev,Todd R. Sandrin Book 2016 Springer International Pub

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期刊全称Applications of Mass Spectrometry in Microbiology
期刊简称From Strain Characte
影响因子2023Plamen Demirev,Todd R. Sandrin
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发行地址Comprehensive treatment of mass spectrometry, a tool at the forefront of bioanalysis that continues to grow.The applications have wide appeal, from practitioners to public health professionals.Contrib
图书封面Titlebook: Applications of Mass Spectrometry in Microbiology; From Strain Characte Plamen Demirev,Todd R. Sandrin Book 2016 Springer International Pub
影响因子.In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome. All of these developments have allowed MS to become a well-established molecular level technology for microorganism characterization. MS has demonstrated its considerable advantage as a rapid, accurate, and cost-effective method for microorganism identification, compared to conventional phenotypic techniques. In the last several years, applications of MS for microorganism characterization in research, clinical microbiology, counter-bioterrorism, food safety, and environmental monitoring have been documented in thousands of publications. Regulatory bodies in Europe, the US, and elsewhere have approved MS-based assays for infectious disease diagnostics. As of mid-2015, more than 3300 commercial MS systems for microorganism identification have been deployed worldwide in hospitals and clinical labs. ..While previous work has covered broader approaches in using MS to characterize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In twelve
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Maximizing the Taxonomic Resolution of MALDI-TOF-MS-Based Approaches to Bacterial Characterization: of counter-bioterrorism strategies, prevention of food contamination, and rapid diagnosis of bacterial infections. This chapter focuses on factors, including culture medium, sample preparation, data acquisition, and data analysis that affect strain-level characterization. In addition, the chapter hi
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Discriminatory Power of MALDI-TOF Mass Spectrometry for Phylogenetically Closely Related Microbial Svel requires rigorous standardization, high spectral quality and the application of adequate sample-processing methods, spectra acquisition procedures, and an optimized data analysis pipeline that includes multivariate fingerprinting approaches. However, these conditions cannot be expected in a rout
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Bacterial Identification at the Serovar Level by Top-Down Mass Spectrometrytion of un-sequenced or newly acquired SNPs and plasmid proteins that may be specific to a given strain without prior knowledge of the sample. Subsequent identification of expressed proteins, serovar-specific biomarkers, and post-translational modifications by top-down LC-MS/MS is integral to rapid
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Stable-Isotope-Based Strategies for Rapid Determination of Drug Resistance by Mass Spectrometrybiomarker masses for phages, initially proliferated in isotopically manipulated growth medium and subsequently grown in microorganisms in growth medium with elements of natural abundance ratios, is monitored and is an indication of drug resistance. Advantages of these assays as well as tools for aut
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Bernhard Kornelius M.A.,Dieter Rothd biomolecules in microbial mixtures or complex biological samples. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is the method of choice for the rapid identification of microorganisms. This chapter concludes with a brief discussion of the selection of MALDI matrices and m
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