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Titlebook: Applications of Mass Spectrometry in Microbiology; From Strain Characte Plamen Demirev,Todd R. Sandrin Book 2016 Springer International Pub

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Sample Preparation Methods for the Rapid MS Analysis of Microorganismsent sample preparation steps used in the context of rapid analysis of microorganisms by mass spectrometry (MS). Sample preparation methods that might eliminate the need for culturing of the target cells (the key rate-limiting step) to obtain detectable signals are important for the rapid analysis of
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Advantages Offered by Proteomic Strategies for Rapid Biodetectioneliable than library matching. Identifications can be made without controlling sample preparation or instrumental variables. One rapidly growing database supports all ionization techniques. In addition, specific biomarkers can be identified for strain identification and forensics. The advantages of
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Bottom-Up Proteomics Methods for Strain-Level Typing and Identification of Bacteria-based methods provide an attractive alternative to well-established microbiological procedures and are characterized by the relatively high speed of acquiring taxonomically relevant information. Bottom-up, shotgun-proteomics techniques allow for high-throughput sequencing of peptides from protease-
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MALDI-TOF MS as a Novel Tool for Dereplication and Characterization of Microbiota in Bacterial Diverionally, this involves first a dereplication step in which all isolates are examined to group those that represent the same taxon in a rapid and cost-effective manner. Dereplication therefore is the assessment of novelty and aims to reduce a large number of isolates to a smaller, nonredundant set fo
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Detection of β-Lactamases and Their Activity Using MALDI-TOF MS, their fast and reliable detection is important for prevention of their uncontrolled spread. Here, we describe two methods based on matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry allowing functional detection of hydrolysis products of β-lactams as well as a
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