Titlebook: Antiviral Resistance in Plants; Methods and Protocol Kappei Kobayashi,Masamichi Nishiguchi Book 2019 Springer Science+Business Media, LLC,

neutralize

Marcelo Fornazin,Luiz Antonio Joiags-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.

破布

BUOY

CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants,gs-CaM (rCML) in . and other Solanaceae plants, including potato (.). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.

Junction

incontinence

隐士

Hong Joo Lee,Minsik Lee,Habin Leeolved have been genetically or biochemically identified in .. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes . mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the r

商议

Hanne Höglund Rydén,Sara Hofmann,Guri Vernenisms has been extensively promoted by virus-induced gene silencing (VIGS) technique, which decreases the transcriptional level of the gene of interest via RNA silencing machinery triggered by the partial gene fragment inserted into a viral vector. This technique has contributed to addressing the fu

Gobble

Ida Lindgren,Csaba Csáki,Anneke Zuiderwijk viruses often results in the breakdown of virus resistance in plants, which leads to a disastrous outcome in agricultural production. Although studies have analyzed the nature of plant virus resistance breakdown, it is still difficult to foresee the breakdown of a given virus resistance gene. In th

convert

Ida Lindgren,Csaba Csáki,Anneke Zuiderwijknt tissues or cells. Thus, with a few exceptions, plant cell extracts are contaminated by vacuole-derived lytic enzymes and unsuitable for biochemical analyses. Here, we describe a method for removing the vacuoles from intact tobacco BY-2 protoplasts and for cell-free translation and replication of

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