Titlebook: Antiviral Resistance in Plants; Methods and Protocol Kappei Kobayashi,Masamichi Nishiguchi Book 2019 Springer Science+Business Media, LLC,

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Ansgar Mondorf,Maria A. Wimmer,Daniel Reisericiency and distribution in a plant based on immunostaining results. In addition, a practical inoculation method that uses a spray gun to apply attenuated viruses to a large number of seedlings is presented.

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Computational Workflow for Small RNA Profiling in Virus-Infected Plants,ication of high quality RNA to guidance for library preparation and sequencing strategies. We report a series of free available tools and programs as well as in-house Perl scripts to perform customized sRNA-seq data mining. Previous bioinformatic background is not required, but experience with basic Unix commands is desirable.

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Ida Lindgren,Csaba Csáki,Anneke Zuiderwijkis chapter, we provide a protocol for evaluating the durability of plant virus resistance gene, which comprises the random mutagenesis of a virus gene, the introduction of the mutagenized gene into a virus context with highly efficient inoculation system, and the efficient screening of virus mutants that can overcome or escape a virus resistance.

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Ida Lindgren,Csaba Csáki,Anneke Zuiderwijkgenomic RNA of positive-strand RNA viruses using the extract of evacuolated protoplasts. We also describe a method for the identification and functional characterization of a plant resistance gene product using this system.

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1064-3745 expertsThis detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel m

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