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Titlebook: Antisense RNA Design, Delivery, and Analysis; Virginia Arechavala-Gomeza,Alejandro Garanto Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable)

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发表于 2025-3-21 18:11:35 | 显示全部楼层 |阅读模式
期刊全称Antisense RNA Design, Delivery, and Analysis
影响因子2023Virginia Arechavala-Gomeza,Alejandro Garanto
视频videohttp://file.papertrans.cn/159/158674/158674.mp4
发行地址This book is open access, which means that you have free and unlimited access.Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementati
学科分类Methods in Molecular Biology
图书封面Titlebook: Antisense RNA Design, Delivery, and Analysis; Virginia Arechavala-Gomeza,Alejandro Garanto Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable)
影响因子This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Antisense RNA Design, Delivery, and Analysis. provides basic knowledge and a large collection of methods to facilitate the workof newcomers to this vibrant and expanding field. .This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics).  DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to e
Pindex Book‘‘‘‘‘‘‘‘ 2022
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2.1.6 References to crystal structure,RNA vaccines against SARS-CoV-2. Another type of nucleic acid therapeutics is antisense oligonucleotides, versatile tools that can be used in multiple ways to target pre-mRNA and mRNA. While some years ago these molecules were just considered a useful research tool and a curiosity in the clinical ma
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2.1.6 References to crystal structure,essary to specifically upregulate target gene translation. Originally identified in the mouse . (antisense Ubiquitin carboxyl-terminal esterase L1) locus, natural SINEUP molecules are oriented head to head to their sense protein coding, target gene (., in this example). Peculiarly, SINEUP is able to
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2.1.1 List of symbols and abbreviations, gene delivery are positively charged to carry negatively charged oligonucleotides. However, excessive positively charged carriers are cytotoxic. Therefore, the complexed oligonucleotide/nanoparticles should be well-examined before the application. In that manner, agarose gel electrophoresis, which
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2.1.1 List of symbols and abbreviations,gh stability and affinity for target sequences. However, in spite of their neutral charge as compared to natural oligonucleotides or phosphorothioate analogs, they still show little permeability for cellular membranes, highlighting the need for effective cytosolic delivery strategies. In addition, t
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