Titlebook: Antisense RNA Design, Delivery, and Analysis; Virginia Arechavala-Gomeza,Alejandro Garanto Book‘‘‘‘‘‘‘‘ 2022 The Editor(s) (if applicable)

耕种

LUDE

腐蚀

2.1.1 List of symbols and abbreviations, we demonstrate that quantitative dot blots in plate format are a better option to determine the absolute contents of a given protein in less than 48 h. The method was optimized for the detection of the Muscleblind-like 1 protein in patient-derived myoblasts treated with a collection of more than 100 experimental oligonucleotides.

防水

FELON

senile-dementia

流动性

Design of Bifunctional Antisense Oligonucleotides for Exon Inclusionously published are listed. We also present methodology of assessing the effects of TOES on exon inclusion in fibroblasts cultured from a SMA patient. The effects of TOES on . exon 7 splicing were validated at RNA level by PCR and quantitative real-time PCR, and at protein level by western blotting.

hieroglyphic

Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrn this evaluation and describe in detail the protocols routinely followed at our institution, one to evaluate the efficacy of skipping at RNA level (nested PCR) and the other the restoration of protein expression (myoblot), which provide good results using equipment largely available to most research laboratories.

floodgate

oblique