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Titlebook: Antibody Phage Display; Methods and Protocol Philippa M. O’Brien,Robert Aitken Book 20021st edition Humana Press 2002

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期刊全称Antibody Phage Display
期刊简称Methods and Protocol
影响因子2023Philippa M. O’Brien,Robert Aitken
视频video
发行地址Includes supplementary material:
学科分类Methods in Molecular Biology
图书封面Titlebook: Antibody Phage Display; Methods and Protocol Philippa M. O’Brien,Robert Aitken Book 20021st edition Humana Press 2002
影响因子The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy—immunotherapy against bacterial infection. As we advance into the new millennium, microbiology and immunology again offer a c- ceptual leap forward as antibody phage display gains increasing acceptance as the definitive technology for monoclonal production and unleashes new - portunities in immunotherapy, drug discovery, and functional genomics. In assembling Antibody Phage Display: Methods and Protocols, we have aimed to produce a resource of real value for scientists who have followed the development of phage display technology over the past decade. The founding principles of phage display have always held an elegant simplicity. We hope that readers will find similar clarity in the technical guidance offered by the book’s contributors. In meeting our objectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont-
Pindex Book 20021st edition
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https://doi.org/10.1007/978-3-662-25909-2anning on individual immobilized antigens (Ags) in order to isolate individual Ag-binding clones. This approach has been successfully applied to numerous purified soluble molecules, yielding high-affinity Abs (.). Selection against almost any soluble Ag is now theoretically feasible.
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Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques, is unknown (e.g., a putative stem cell or tumor-specific marker) or because the process of purification destroys its native conformation (e.g., in the case of some integral membrane proteins). In such experimental systems, methods that select phage-displayed Ig directly on intact cell surfaces are required.
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