Titlebook: Animal Models of Acute Neurological Injuries II; Injury and Mechanist Jun Chen,Xiao-Ming Xu,John H. Zhang Book 2012 Springer Science+Busine

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Application of Zymographic Methods to Study Matrix Enzymes Following Traumatic Brain Injuryetalloproteinases (MMPs), specifically those investigating the role of gelatinases (MMP-2, MMP-9), gelatin zymography is used to assess relative changes in enzyme activity. The advantages of this method lie in its gel separation of pro and active enzyme and the option for generation of semiquantitat

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Rodent Behavioral Assessment in the Home Cage Using the SmartCage™ System provided unprecedented opportunities for new drug development. To facilitate investigation of diverse behavioral processes, we have developed a home cage monitoring system for automated rodent neurobehavioral phenotypic analysis and higher-throughput in vivo drug screening. This system termed Smart

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Assessments of Cognitive Function Following Traumatic Brain Injurysociated with traumatic brain injury (TBI) in clinic. TBI in humans results in a myriad of functional deficits. However, cognitive deficits, particularly memory and information processing speed, are the most prominent and persistent features following brain injury of any severity. Experimental model

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1949-2448 ernative methods that have their own special utility.Feature.The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal co

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https://doi.org/10.1057/9780230504349 miRNAs. TaqMan real-time PCR is a specific, sensitive, and accurate method for assessing miRNA expression, allowing direct analysis in a single cell without the need for nucleic acid purification. This chapter introduces a brief history of Taqman real-time PCR, required materials and procedures, software, and statistical analysis.

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German Monetary Union and Currency Reformaviors, basic associative learning, relational learning, short-term memory, long-term memory, and problem-solving strategy are among the domains that can be assessed with the data collected. After initial characterization of the model, a subset of the tests may provide a more efficient battery for therapeutic studies using that model.

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MicroRNA Expression Profiling of Rat Blood and Brain Tissues: TaqMan Real-Time PCR MicroRNA Assays miRNAs. TaqMan real-time PCR is a specific, sensitive, and accurate method for assessing miRNA expression, allowing direct analysis in a single cell without the need for nucleic acid purification. This chapter introduces a brief history of Taqman real-time PCR, required materials and procedures, software, and statistical analysis.

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Assessment of Cognitive and Sensorimotor Deficitsaviors, basic associative learning, relational learning, short-term memory, long-term memory, and problem-solving strategy are among the domains that can be assessed with the data collected. After initial characterization of the model, a subset of the tests may provide a more efficient battery for therapeutic studies using that model.