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Titlebook: Animal Endo-SiRNAs; Methods and Protocol Andreas Werner Book 2014 Springer Science+Business Media New York 2014 C. elegans.Dicer molecule.S

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期刊全称Animal Endo-SiRNAs
期刊简称Methods and Protocol
影响因子2023Andreas Werner
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Animal Endo-SiRNAs; Methods and Protocol Andreas Werner Book 2014 Springer Science+Business Media New York 2014 C. elegans.Dicer molecule.S
影响因子.Animal Endo-SiRNAs: Methods and Protocols. presents a variety of approaches to investigate endo-siRNAs. These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Animal Endo-SiRNAs: Methods and Protocols. contains practical tips that are absent in standard lab manuals..
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Craig H. Faunce,Craig A. Laymanethod yields pure fractions of CBs, and it is robust, reproducible and does not require special equipment or abundant starting material. The CB is packed with large amounts of RNA, especially small RNAs. Isolation of the CBs provides a tool to enrich these RNA species.
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Ecological Crisis Through a Social Lens,tly detecting endo-siRNA levels in bulk, as well as an optimized qPCR method for detecting the effect of endo-siRNAs on gene targets. Intriguingly, the loss of endo-siRNAs frequently results in enhanced experimental RNAi. Thus, we also present an optimized method to assess the indirect impact of endo-siRNAs on experimental RNAi efficiency.
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The empirical value of diversity measures,orthern blotting using DIG-labelled RNA probes. Moreover, it includes a strategy to design and generate cheap hybridization probes with greatly increased sensitivity. These methods may be used as a simple and robust protocol for nonradioactive detection of small RNAs or be combined with other strategies to potentially enhance signal intensity.
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The Economic System and the Environmentalso provide guidance for analysis of primary bisulfite sequencing data and interpretation of the methylation status using the Web-based bisulfite sequencing DNA methylation (BISMA) analysis. This refined and reproducible assay can be performed even using a small amount of genomic DNA and is suitable for the analysis of clinical tissue samples.
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