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Titlebook: Animal Coronaviruses; Leyi Wang Book 2016 Springer Science+Business Media New York 2016 Coronaviridae.Reverse genetics.Molecular diagnosti

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Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.
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PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Genetocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.
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Ecocardiografia per l‘intensivistaethod uses chicken infectious bronchitis coronavirus (IBV) as the coating antigen based on antigenic cross-reactivity between TCoV and IBV. The other method relies on a recombinant TCoV nucleocapsid protein. Both methods are useful for serological diagnosis of TCoV infection in the turkey flocks.
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https://doi.org/10.1007/978-88-470-1384-1tocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.
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Ecocardiografia per l‘intensivistatained for TCoV. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene is obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene is obtained for BCoV.
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Virus Neutralization Assay for Turkey Coronavirus Infectionum that can inhibit TCoV infection in 50 % of embryonated turkey eggs. Virus neutralization assay for TCoV is useful to monitor the immune status of turkey flocks infected with TCoV for the control of the disease.
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