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Titlebook: Animal Coronaviruses; Leyi Wang Book 2016 Springer Science+Business Media New York 2016 Coronaviridae.Reverse genetics.Molecular diagnosti

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发表于 2025-3-21 16:10:19 | 显示全部楼层 |阅读模式
期刊全称Animal Coronaviruses
影响因子2023Leyi Wang
视频video
发行地址Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry.Provides step-by-step detail essential for reproducible results.Contains key notes and implem
学科分类Springer Protocols Handbooks
图书封面Titlebook: Animal Coronaviruses;  Leyi Wang Book 2016 Springer Science+Business Media New York 2016 Coronaviridae.Reverse genetics.Molecular diagnosti
影响因子.This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the .Springer Protocols Handbooks. series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. .Practical and authoritative, .Animal Coronaviruses. serves as an ideal reference for researchers examining a wide variety of coronavirus species in the .Coronaviridae..
Pindex Book 2016
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书目名称Animal Coronaviruses读者反馈学科排名




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发表于 2025-3-21 21:09:29 | 显示全部楼层
Il ventricolo destro e l’arteria polmonareplete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA dependant RNA polymerase.
发表于 2025-3-22 04:00:11 | 显示全部楼层
Ecocardiografia per l‘intensivista spike gene (S2) of TCoV. The assay is highly specific and sensitive and can quantitate between 10. and 10. copies/mL of viral genome. It is useful in monitoring the progression of TCoV-induced atrophic enteritis in the turkey flocks.
发表于 2025-3-22 07:01:11 | 显示全部楼层
Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virusplete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA dependant RNA polymerase.
发表于 2025-3-22 09:31:37 | 显示全部楼层
发表于 2025-3-22 13:51:12 | 显示全部楼层
1949-2448 ntial for reproducible results.Contains key notes and implem.This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assay
发表于 2025-3-22 17:42:46 | 显示全部楼层
Ecocardiografia per l‘intensivistaogalactopyranoside (IPTG). The expressed N protein inclusion body is extracted and purified by chromatography on nickel-agarose column to near homogeneity. The protein recovery can be 10 mg from 100 ml of bacterial culture. The purified N protein is a superior source of TCoV antigen for antibody-capture ELISA for detection of antibodies to TCoV.
发表于 2025-3-22 23:07:22 | 显示全部楼层
Cenni di fisica degli ultrasuoni and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.
发表于 2025-3-23 02:59:45 | 显示全部楼层
Ecocardiografia per l‘intensivistance. It is difficult to diagnose FIP, since the viruses cannot be distinguished using serological or virological methods. Recently, genetic techniques, such as RT-PCR, have been conducted for FIP diagnosis. In this chapter, the reliability of RT-PCR and procedures used to determine FCoV infection as part of antemortem FIP diagnosis is described.
发表于 2025-3-23 07:17:44 | 显示全部楼层
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