Titlebook: Animal Cell Culture; Mohamed Al-Rubeai Book 2015 Springer International Publishing Switzerland 2015 Animal Cell Culture.Biologics.Biopharm

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Transient Recombinant Protein Expression in Mammalian Cells,s review describes the different methods for introducing foreign DNA into suitable mammalian cells with either viral or non-viral vectors. Particular emphasis is given to non-viral transient transfection which represents meanwhile the most prominent variant due to recent progress in the resulting pr

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RLS898

Bioreactors for Mammalian Cells,ands. Numerous bioreactors and cultivation systems for cell culture, either for production of biopharmaceuticals or for tissue engineering, have been developed. Due to the special characteristics of these cells specific solutions are required. Selection of a suitable type of cell culture bioreactor

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Mass Transfer and Mixing Across the Scales in Animal Cell Culture,. Oxygen in solution has to be continuously provided because of its low solubility in aqueous solution which is continually being utilised by the growing cells (at the current time, reaching a cell density of ~10. cells mL. in bioreactors up to 25 m.). Such a process requires a certain specific powe

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Serum and Protein Free Media,f variability in composition, cost but particularly the potential for contamination with viruses or prions has been the driver for the substitution of serum with a more defined and animal-component free media. For some cell lines substitution with just a few simple ingredients can provide an effecti

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