Titlebook: Animal Cell Biotechnology; Methods and Protocol Nigel Jenkins Book 19991st edition Springer Science+Business Media New York 1999

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书目名称Animal Cell Biotechnology影响因子(影响力)




书目名称Animal Cell Biotechnology影响因子(影响力)学科排名




书目名称Animal Cell Biotechnology网络公开度




书目名称Animal Cell Biotechnology网络公开度学科排名




书目名称Animal Cell Biotechnology被引频次




书目名称Animal Cell Biotechnology被引频次学科排名




书目名称Animal Cell Biotechnology年度引用




书目名称Animal Cell Biotechnology年度引用学科排名




书目名称Animal Cell Biotechnology读者反馈




书目名称Animal Cell Biotechnology读者反馈学科排名

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Adaptation of Mammalian Cells to Growth in Serum-Free Medialtivation in suspension culture, without serum, and at high cell densities. Such phenotypes are useful for the production of recombinant proteins and monoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread

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OGLE

Optimizing Gene Expression in Mammalian Cellsalian cells are essential to perform such modifications correctly, and efficient methods have been established to transduce or transfect them, based on either viral or synthetic vectors, or combinations of both. Several parameters affect the efficiency of expression of the transgene, such as the nat

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Immortalization Strategies for Mammalian Cellsescence. However, when the control mechanisms which limit life span are modified cells can become immortalized i.e. have an unlimited growth potential (.). One such way of subverting these control mechanisms is to express an immortalizing oncogene in normal cells. These proteins override the normal

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变量

Cell Bank Preparation and Characterizationerties of a cell line or of a product derived from it, it is, therefore, necessary to cryopreserve and characterize a reference stock of cells. Thereafter, throughout a research, development, or manufacturing program cell cultures are discarded after a defined passage interval and the cryopreserved

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Cell Counting and Viability Measurementst method is by cell counting at regular intervals. In routine cultures this would be performed once a day, which corresponds to the approximate doubling time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts woul