Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf Pörtner Book 2007Latest edition Humana Press 2007 DNA.Downstream Processing.Expression

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https://doi.org/10.1007/978-3-030-17034-9h and fed-batch systems. A simplified network of mammalian cell metabolism is used to illustrate the flux estimation procedure, and the steps leading up to the consistency index determination are presented. If gross measurement errors are detected, a technique for determining the source of gross mea

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Anastasiia Grynko,Katerina Tsetsuraneity of cell populations, permitting a rapid and sensitive cell analysis. In this chapter, various flow cytometric applications in cell culture are described for cell lines grown on a laboratory scale, but the principles can also be employed for the monitoring of cell lines up to the large industrial scale.

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Ralf PörtnerChapters written by world-renowned experts.Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors.Provid

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https://doi.org/10.1007/978-1-349-23366-3e sciences. Multiply attenuated viral vector systems, which are able to transfer heterologous DNA or RNA into almost any cell phenotype as well as in vivo, emerged as the most efficient transgene delivery systems in modern biology. We provide a comprehensive technical overview on the most commonly u

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Social Policy in a Post-Socialist Vacuuml diseases. Antibody technology has been revolutionized by the hybridoma technology for rnAb production described by Köhler and Milstein in 1975 (.). In the last 30 yr a number of genetically engineered antibody constructions have emerged, including chimeric and human-like antibodies as well as diff