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Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf Pörtner Book 2007Latest edition Humana Press 2007 DNA.Downstream Processing.Expression

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Measurement of Apoptosis in Cell Cultureare several biochemical and cytometric techniques of varying complexity that have been developed to detect cellular and subcellular changes occurring during apoptosis. For all procedures, cells can be obtained from either monolayer or suspension culture.
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https://doi.org/10.1057/9781137385130w to handle the CeLLine, the miniPerm, and the Wave. In our experience, the methods described for a Chinese hamster ovary (CHO) suspension cell line can also be successfully applied to other animal suspension cells, such as insect cells (Sf-9) and human embryogenic kidney cells (HEK-293 EBNA cells).
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Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragmentsengineering and production methods and describe standard protocols for producing a hybridoma cell line, the building up of transfectomas, and a suitable bacterial expression system for the production of antibodies and antibody fragments established in our laboratory.
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