Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under

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The Dictionary of Drugs: Chemical Dataps) that include versus exclude a particular alternative RNA segment. The ratio measurement to study alternative splicing regulation can be confounded when alternative isoforms undergo differential RNA decay, for example, nonsense-mediated mRNA decay (NMD). Isoform-centric quantification is more inf

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R. G. Mirimanov,N. E. Sirotina,V. I. Neymanell/tissue types and disease, and what roles different AS events play. To facilitate AS research, we have created the computational . framework, which comprises a series of complementary software and resources that we describe in this chapter. The . framework is especially designed to aid biomedical

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https://doi.org/10.1007/978-3-642-98090-9 transcriptome-wide sequencing technologies highlight the remarkable extent of this regulation in metazoans and allow for RNA isoforms to be profiled in increasingly small biological samples and with a growing confidence. Understanding biological functions of sample-specific transcripts is a major c

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Cytology

Part III Food Definitions and Formulationsngle-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscrip

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https://doi.org/10.1007/978-1-4419-9713-5and the mechanisms controlling alternative splicing in specific cellular contexts. Reporters that recapitulate alternative splicing patterns of endogenous transcripts have served as excellent tools for dissecting regulatory mechanisms of splicing. In this chapter, we describe a two-color fluorescent