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Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under

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发表于 2025-3-21 17:32:04 | 显示全部楼层 |阅读模式
期刊全称Alternative Splicing
期刊简称Methods and Protocol
影响因子2023Peter Scheiffele,Oriane Mauger
视频video
发行地址Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under
影响因子This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful .Methods in Molecular Biology. series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Alternative Splicing: Methods and Protocols. serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects..
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Alireza Bahadori,Scott T. Smithe fluorescent probes that are highly specific to their RNA target. To facilitate probe design, we have created anglerFISH, a user-friendly command-line based pipeline. In this chapter, we present how to perform a smFISH experiment using user-designed and labeled probes.
发表于 2025-3-22 04:50:35 | 显示全部楼层
Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data,xons of length up to 30 nt, require specialized computational workflows. Here, we describe MicroExonator, a reproducible computational workflow for microexon splicing analysis using bulk or single-cell RNA-seq data.
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发表于 2025-3-22 14:47:08 | 显示全部楼层
R. G. Mirimanov,N. E. Sirotina,V. I. Neymanregulatory conservation among species (.), and (d) help with the biological interpretation of the results, and, ultimately, with the identification of interesting AS events to design wet-lab experiments (. and .).
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https://doi.org/10.1007/978-1-4419-9713-5ynthetic splicing factors using solution state NMR spectroscopy. This approach allowed us to validate direct interactions between splicing regulators and U1 snRNP and could also be useful for the screening of small molecules acting on splicing regulation.
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发表于 2025-3-23 08:33:19 | 显示全部楼层
Computational Analysis of Alternative Splicing Using , and the , Framework,regulatory conservation among species (.), and (d) help with the biological interpretation of the results, and, ultimately, with the identification of interesting AS events to design wet-lab experiments (. and .).
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