Titlebook: Allergy; Methods and Protocol Penny Lympany,Meinir G. Jones Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer

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Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive Eay immune-regulatory roles in human innate and adaptive immunity. Protocols have been individually optimized to yield ultrasensitive limits of detection and quantification. Major factors enhancing immunoassay sensitivity, precision, and reproducibility, as well as key pitfalls in assay design and execution, are critically reviewed.

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Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Pen presentation. The method for isolating T cells and monocytes from peripheral blood mononuclear cells (PBMC) and the stimulation of T cells to proliferate and produce cytokines by MDC are outlined in this chapter. The method can be applied to the functional studies of T cells and DC in other diseases.

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Book 2019Latest editionous protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and charac

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Abhijeet Lokras,Camilla Foged,Aneesh Thakurdization of allergen extracts is the use of standards..This chapter describes the principles for standardization of allergen extracts to be used by research laboratories. Other chapters in this volume describe in vitro methods in detail.