Titlebook: Allergy; Methods and Protocol Penny Lympany,Meinir G. Jones Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer

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K. C. Vora,Kartik E. Gurnule,S. Venkateshiated with significant increases in allergen-specific IgG. antibodies. Natural immune tolerance observed in beekeepers and cat owners has also been associated with elevated levels of bee venom and cat allergen-specific IgG., respectively. Functionally, allergen-specific IgG. has been shown to reduce

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https://doi.org/10.1007/978-3-319-02892-7identification. Western blotting using serum allows us to identify a protein within an allergenic extract that specifically binds to a serum antibody, immunoglobulin E. This specific IgE binding protein can then be detected with a highly sensitive chemiluminescence detection substrate..Proteins dete

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Design and Deploy an Identify Solution,Cell-tracker fluorescent dye labeling is widely used for investigating antigen-specific immune responses in vitro and in vivo. Here we describe a development of this technique—the use of dual-cell-tracker dye staining for the identification and characterization of the responses of different T-cell subsets to peanut proteins in vitro.

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The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-SpeCell-tracker fluorescent dye labeling is widely used for investigating antigen-specific immune responses in vitro and in vivo. Here we describe a development of this technique—the use of dual-cell-tracker dye staining for the identification and characterization of the responses of different T-cell subsets to peanut proteins in vitro.

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/153494.jpg

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Allergy978-1-4939-9591-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/979-8-8688-0239-3en presentation. The method for isolating T cells and monocytes from peripheral blood mononuclear cells (PBMC) and the stimulation of T cells to proliferate and produce cytokines by MDC are outlined in this chapter. The method can be applied to the functional studies of T cells and DC in other diseases.

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Topics on Selective Catalyst Reductionhed to absorb excess reagents. The membrane is stripped with specific antibodies against soy allergens in this case (test line) and a goat anti-rabbit IgG antibody (control line). The strip is exposed to a mix of sample and gold-conjugated specific antibody. Colored bands are read out visually or by densitometry.